|
KMID : 1007520130220020507
|
|
Food Science and Biotechnology
2013 Volume.22 No. 2 p.507 ~ p.513
|
|
|
Cyanidin-3-O-(2"-xylosyl)-glucoside, an Anthocyanin from Siberian ginseng (Acanthopanax senticosus) Fruits, Inhibits UVB-induced COX-2 Expression and AP-1 Transactivation
|
|
Jung Sung-Keun
Lim Tae-Gyu
Seo Sang-Gwon
Lee Hyong-Joo
Hwang Young-Sun
Choung Myoung-Gun
Lee Ki-Won
|
|
|
Abstract
|
|
|
|
Cyanidin-3-O-(2"-xylosyl)-glucoside (C-3-O- (2"-xylosyl)-G) was analyzed as an active constituent from the fruit of Siberian ginseng (Acanthopanax senticosus) using a HPLC diode array detection-electrospray ionization/ mass spectrometry analysis system and the effect of C-3- O-(2’’-xylosyl)-G on UVB-induced inflammatory signaling in JB6 P+ cells was investigated. C-3-O-(2"-xylosyl)-G inhibited UVB-induced cyclooxygenase (COX)-2 expression and promoter binding activity in JB6 P+ cells and JB6 P+ cells stably transfected with the COX-2 luciferase reporter plasmid. It inhibited both the UVB-induced activator protein-1 (AP-1) and nuclear factor (NF)-κB transactivation in JB6 P+ cells stably transfected with the AP- 1 and NF-κB luciferase reporter plasmids. Additionally, C- 3-O-(2"-xylosyl)-G significantly suppressed UVB-induced upregulated phosphorylation of c-Jun N terminal kinase, MEK/extracellular signaling kinase, and mitogen activated protein kinase kinase (MAPKK) 3/6 in JB6 P+ cells. These results indicate that C-3-O-(2"-xylosyl)-G may be a promising chemopreventive material that acts by suppressing COX-2 expression and AP-1 and NF-KB transactivation and JNK, MAPKK3/6, and MEK/ERK1/2 phosphorylation.
|
|
|
KEYWORD
|
|
|
cyanidin-3-O-(2"-xylosyl)-glucoside, Acanthopanax senticosus, COX (cyclooxygenase)-2, activator protein-1 (AP-1), nuclear factor (NF)-KB
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|